Samoa Digital Library

Microbial Contamination Survey of Environmental Fresh and Saltwater Resources of Upolu Island, Samoa

Show simple item record

dc.contributor.author Ochsenkuhn, Michael A
dc.contributor.author Amosa, Patila
dc.contributor.author Fei, Cong
dc.date.accessioned 2023-06-16T02:19:52Z
dc.date.available 2023-06-16T02:19:52Z
dc.date.issued 2021-10-25
dc.identifier.citation Ochsenkühn, M.A.; Fei, C.; Bayaara, O.; Romeo, E.; Amosa, P.; Idaghdour, Y.; Goldstein, G.; Bromage, T.G.; Amin, S.A. Microbial Contamination Survey of Environmental Fresh and Saltwater Resources of Upolu Island, Samoa. Environments 2021, 8, 112. https://doi.org/10.3390/ environments8110112 sm
dc.identifier.uri https://doi.org/10.3390/environments8110112
dc.identifier.uri ${sadil.baseUrl}/handle/123456789/3700
dc.description 15 p. (pdf ) ( This article belongs to the Special Issue Monitoring and Management of Inland Waters) sm
dc.description.abstract Analysis of microbe diversity in freshwater resources and nearshore seawater samples of Upolu Island was performed to investigate the distribution of harmful bacteria. For this, 124 samples were collected from 23 river systems, two volcanic lakes, and 45 locations inside and outside the barrier reef of Upolu Island, Samoa. Physicochemical parameters for general water quality, detection of coliform bacteria and 16S rRNA amplicon sequencing were performed on all samples. Fecal indicator bacteria (FIB) testing indicated a wide distribution of coliform bacteria in all sampled freshwater sites with evidence of fecal coliform in most locations. Importantly, evidence of coliform bacteria was found in most seawater samples inside and along the reef, apart from those samples taken ~20 km offshore. Illumina 16S rRNA amplicon sequencing of the V4 hypervariable region confirmed the presence of various types of harmful bacterial species, namely from the Enterobacteriaceae, Enterococcae, Streptococcaceae, and Vibrionacea families. By combining the sensitivity of FIB testing and next-generation sequencing, we were able to show the extent of potential contaminations in fresh and seawater samples and simultaneously identify the potential pathogenic bacterial genera present. The wide distribution of potential harmful bacteria from river runoff or direct sewage dumping has an impact on human health, leading to many skin and intestinal diseases, and is potentially detrimental to coral reef community health. sm
dc.description.sponsorship UNDP & NYU Abu Dhabi sm
dc.language.iso en sm
dc.publisher Enviroments sm
dc.relation.ispartofseries Environments 2021;8, 112
dc.subject microbes sm
dc.subject freshwater sm
dc.subject saltwater sm
dc.subject diversity sm
dc.subject fecal sm
dc.subject biosecurity sm
dc.subject Samoa sm
dc.subject coral reefs sm
dc.title Microbial Contamination Survey of Environmental Fresh and Saltwater Resources of Upolu Island, Samoa sm
dc.type Article sm


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account